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Journal of Indian Association of Pediatric Surgeons
     Journal of Indian Association of Pediatric Surgeons
Official journal of the Indian Association of Pediatric Surgeons         
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 ORIGINAL ARTICLE
Year : 2013  |  Volume : 18  |  Issue : 2  |  Page : 66-68

Calretinin immunohistochemistry: A new cost-effective and easy method for diagnosis of Hirschsprung's disease


1 Department of Pediatric Surgery, Niloufer Hospital for Women and Children, Osmania Medical College, Hyderabad, India
2 Department of Pathology, Niloufer Hospital for Women and Children, Osmania Medical College, Hyderabad, India

Correspondence Address:
Lavanya Kannaiyan
Department of Pediatric Surgery, Niloufer Hospital for Women and Children, Osmania Medical College, Hyderabad
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-9261.109355

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Aim: To evaluate the efficacy of calretinin immunostaining in diagnosing Hirschsprung's disease (HD). Materials and Methods: Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination. Results: Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining. Conclusions: Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available.






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